Lys20p and Lys21p

Catalogue # MCA-31F5

Monoclonal Antibody 31F5 to yeast Lys20p and Lys21p.

The Immunogen: Lys20p and Lys21p are two isozymes of yeast homocitrate synthase. Their systematic names YDL182W and YDL131W respectively. Lys20p is of molecular size 47.1 kDa and Lys21p is 48.6 kDa; they are ~90% identical at the amino acid level. MCA-31F5 detects both proteins as a closely spaced doublet on immunoblots. Both proteins are localized to the nucleoplasm of the nucleus (1).

Diagram of Domain Structure: Generated from sequence of yeast Lys20p (top) and Lys21p (bottom) with the SMART program from EMBLHydroxymethylglutaryl-CoA lyase (HMGL) like domain which is found in a diverse set of enzymes including various aldolases, pyruvate carboxylases and homocitrate synthases. Scale is number of amino acids; in Heidleberg. The block marked "PFAM HMGL-like" corresponds to the

Suggestions for use: For immunoblots of yeast protein samples, use MCA-31F5 diluted 1/10,000 to 1/50,000 followed by chemiluminescent detection (ECL). For non-ECL western detection methods, try MCA-31F5 diluted 1/1,000 to 1/5,000. For immunofluorescence on yeast cells, use MCA-31F5 diluted 1/1,000 to 1/10,000 (we routinely dilute MCA-31F5 at 1/5,000). Antibody preparation contains 10mM sodium azide preservative (Link to here for Material Safety Data Sheet). Avoid repeated freezing and thawing, store at 4°C or -20°C.

References:

Chen, S., J. E. Dove, J. S. Brockenbrough, and J. P. Aris. (1997) Homocitrate synthase is located in the nucleus in the yeast Saccharomyces cerevisiae. J. Biol. Chem. 272:10839-10846.

Comprehensive Yeast Genome Database (CYGD) Entries: View, Lys21p press here. Lys20p press

Yeast Genome Database (Stanford) Entries: Lys20p Press here, Lys21p press here.

Limitations: This product is for research use only and is not approved for use in humans or in clinical diagnosis.

Availability and Price: Available for shipping now, $200 US per aliquot of 100ml of filtered and sterile ascites, enough for hundreds of experiments.